医学创新论坛第31期

时间：2024年7月9日（周二）上午10:00

地点：首都医科大学基础科研楼北楼1322会议室

主持人：李国民 特聘研究员

        首都医学科学创新中心

主讲人：任捷 研究员

中国科学院北京基因组研究所

TITLE：Nascent RNA at the crossroads of transcription and replication

ABSTRACT

Transcription‒replication collisions have a profound impact on genome and epigenome stability, which in turn plays a prominent role in cancer and aging. To address this complex issue, our laboratory focuses on revealing the intricate network of pathways involved in replication fork protection and restart, RNA polymerase release and reactivation, damage repair, and epigenetic inheritance, which are coordinated by novel aspects of RNA-mediated chromatin regulation and respond to this conflict. By revealing the precise molecular mechanisms that prevent transcription-induced replication stress, our research aims to provide a framework for understanding the molecular basis of cancer and aging. Furthermore, we leverage the advancements in omic tools to translate this knowledge into applications in precision medicine. The pertinent findings have been published in Cell, Nature, Cell Research, Cell Stem Cell, Nature Medicine, Nature Aging and Molecular Cell. 

In this talk, I will focus on our mechanistic studies, providing insights into the roles of nascent RNA in transcription termination through modifications and structures, with implications for genome stability and diseases.

SELECTED PAPERS

1. Hao JD, Liu QL, Liu MX, Yang X, Wang LM, Su SY, Xiao W, Zhang MQ, Zhang YC, Zhang L, Chen YS, Yang YG, Ren J. DDX21 mediates co-transcriptional RNA m6A modification to promote transcription termination and genome stability. Mol Cell. 2024 May 2;84(9):1711-1726.e11.
2. Yang X, Liu QL, Xu W, Zhang YC, Yang Y, Ju LF, Chen J, Chen YS, Li K, Ren J, Sun Q, Yang YG. m6A promotes R-loop formation to facilitate transcription termination. Cell Res. 2019 Dec;29(12):1035-1038.
3. Jing Y, Jiang X, Ji Q, Wu Z, Wang W, Liu Z, Guillen-Garcia P, Esteban CR, Reddy P, Horvath S, Li J, Geng L, Hu Q, Wang S, Belmonte JCI, Ren J, Zhang W, Qu J, Liu GH. Genome-wide CRISPR activation screening in senescent cells reveals SOX5 as a driver and therapeutic target of rejuvenation. Cell Stem Cell. 2023 Nov 2;30(11):1452-1471.e10.